PNC-28 Peptide Overview

PNC-28 is a synthetic 27-amino-acid chimeric peptide designed as a truncated analog of PNC-27, containing a shortened HDM-2 binding domain derived from the p53 tumor suppressor (residues 17–26) fused to a cell-penetrating peptide (CPP) sequence from Antennapedia. By omitting the first five amino acids of the PNC-27 p53 domain, PNC-28 retains selective cytotoxic activity against cancer cells while providing a distinct structure-activity profile.

This membrane-active peptide selectively targets HDM-2 proteins on cancer cell membranes, promoting oligomeric pore formation and membrane permeabilization via penetratin-mediated translocation. PNC-28 is widely used as a molecular tool to study anticancer peptide mechanisms, selective tumor targeting, and membrane disruption pathways.

Research Applications

PNC-28 is intended for in vitro research applications and has been employed in studies including:

HDM-2 / p53 protein interaction analyses
Membrane permeabilization and pore formation assays
Selective necrotic death in pancreatic, ovarian, and breast cancer cell lines
Structure-activity relationship investigations of truncated anticancer peptides
Penetratin-mediated cellular uptake and membrane disruption studies

Product Quality and Manufacturing

PNC-28 is supplied as pharmaceutical-grade lyophilized powder, manufactured in the USA under GMP-compliant conditions. Each batch undergoes third-party verification for molecular identity, purity (≥99% HPLC), sterility, and endotoxin levels to ensure research reproducibility and quality.

SynoPept provides verified documentation, stable storage conditions, and rapid shipping to support controlled experimental research.

For in vitro research use only. Not intended for diagnostic, therapeutic, or clinical applications.